Journal: Stem Cell Research & Therapy

Article Title: Chemotaxis-driven disease-site targeting of therapeutic adult stem cells in dystrophic epidermolysis bullosa

doi: 10.1186/s13287-016-0388-y

Figure Lengend Snippet: Analysis of chemokines in DEB-associated blister fluids and chemokine receptors on the surface of mADSC. a Chemokine profile in blister fluids of Col7a1 –/– ( DEB ) and Col7a1 f lNeo/f lNeo ( Hypo DEB ) mice conducted using Proteome Profiler™ Mouse Chemokine Antibody Array. Blister fluids were collected from blister-affected skin (paw, armpit, neck, and abdomen) of newborn mice. Spot intensities were normalized based on background levels and positive control signals. Data shown as mean ± SD from three independent arrays ( p > 0.05). b Surface expression of chemokine receptors in primary mADSC on early passage (passages 2–3) detected by flow cytometry. Light gray curves , control antibody signal; dark shaded curves , specific antibody signal. Histograms are representative examples of chemokine receptor expression profiles obtained from five independent mADSC preparations. c Quantitation of flow cytometry results for expression of the chemokine receptors in primary mADSC. Data shown as mean ± SD of cell percentage. DEB dystrophic epidermolysis bullosa

Article Snippet: Proteome ProfilerTM Mouse Chemokine Antibody Array (R&D Systems, Minneapolis, MN, USA) was employed to assay blister fluid samples derived from Col7a1 –/– and Col7a1 f lNeo/f lNeo mice, respectively.

Techniques: Ab Array, Positive Control, Expressing, Flow Cytometry, Control, Quantitation Assay

Journal: Stem Cell Research & Therapy

Article Title: Chemotaxis-driven disease-site targeting of therapeutic adult stem cells in dystrophic epidermolysis bullosa

doi: 10.1186/s13287-016-0388-y

Figure Lengend Snippet: Characterization of cell surface chemokine receptors on mADSC after treatment with selected growth factors and cytokines. a FACS-based quantitation of the chemokine receptor-positive populations of mADSC under different culture conditions. Selected chemokine receptors identified on the surface of the cells indicated above the profiles, treatment conditions indicated on the panel: 1× confluent culture, 3× high-density culture. Treatments that increase populations of chemokine receptor-positive cells are indicated to the right of the profiles. b Percentage of receptor-positive cells in confluent cultures. Treatment conditions are shown to the right of the graphs. Data shown as mean ± SD from three independent experiments. * p < 0.05 compared with control (1×) cells

Article Snippet: Proteome ProfilerTM Mouse Chemokine Antibody Array (R&D Systems, Minneapolis, MN, USA) was employed to assay blister fluid samples derived from Col7a1 –/– and Col7a1 f lNeo/f lNeo mice, respectively.

Techniques: Quantitation Assay, Control

Journal: Biomedicines

Article Title: Bacterial Outer Membrane Vesicles Promote Lung Inflammatory Responses and Macrophage Activation via Multi-Signaling Pathways.

doi: 10.3390/biomedicines11020568

Figure Lengend Snippet: Figure 4. OMVs treatment in macrophages induces pro-inflammatory cytokines and chemokines. (A,B) BMDM were treated with OMVs at the indicated doses, after 24 h, mouse inflammation and autoimmune arrays were used to screen for candidates involved in OMV-stimulated inflammatory responses. Panel (A) shows upregulated chemokines and panel (B) shows downregulated chemokines. To visually present the up- and down-regulation resulted from OMV treatment, control groups were normalized to 1. (C) A mouse chemokine array was performed to screen for alterations in chemokine levels resulted from equal amounts of EXO or OMV treatment in MHS. Cells were treated with 0.01 µg/mL OMV or EXO for 24 h. (C) Representative graph showing 7 of the 25 chemokines profiled by the chemokine array. Data points were analyzed using two-tailed unpaired Student’s t-test to compare a treatment group to the control group; Mean ± SD; * p ≤0.005, ** p ≤0.001, *** p ≤0.0005, **** p ≤0.001 versus the control (Negative control EB treated); # p ≤0.005, ## p ≤0.001, #### p ≤0.001 vs. Exosome-treated.

Article Snippet: To check chemokine expression in MHS cells treated with exosomes or OMVs, a Proteome ProfilerTM Antibody Arrays–Mouse Chemokine Array (R&D Systems, Minneapolis, MN, USA) was used.

Techniques: Control, Two Tailed Test, Negative Control

Journal: Virus Research

Article Title: Cowpox virus but not Vaccinia virus induces secretion of CXCL1, IL-8 and IL-6 and chemotaxis of monocytes in vitro

doi: 10.1016/j.virusres.2012.11.013

Figure Lengend Snippet: Average change of cytokine gene expression in infected cells compared to non-infected cells. Cytokine gene expression was analysed by TaqMan ® real-time PCR and normalized to cMyc gene expression (ΔcT). The fold change values were calculated using the ΔΔcT method by subtraction of average ΔcT values obtained from mock-infected cells from those of infected cells. a As CSF2 expression was not detectable in non-infected cells, CSF2 expression in VACV-infected cells was used as reference for ΔΔcT calculation of CPXV- (strain BR) or MPXV-infected cells.

Article Snippet: Cell culture supernatants were screened with the Proteome ProfilerTM Human Cytokine Antibody Array, Panel A (R&D Systems).

Techniques: Gene Expression, Infection, Real-time Polymerase Chain Reaction, Expressing

Journal: Virus Research

Article Title: Cowpox virus but not Vaccinia virus induces secretion of CXCL1, IL-8 and IL-6 and chemotaxis of monocytes in vitro

doi: 10.1016/j.virusres.2012.11.013

Figure Lengend Snippet: Cytokine secretion by infected cells compared to non-infected cells. Relative quantification of pro-inflammatory cytokines in cell culture supernatants of VACV- or CPXV-(strains BR or CPXV HumKre08/1) infected or mock-infected HeLa cells, as measured via a protein array capable of detecting 36 different cytokines and chemokines simultaneously. The signal intensities (rlu, relative luminescence units) indicated are calculated by using the intensities of the corresponding protein spots after background correction and normalization of the intensities according to the intensity of the positive control. Shown are the 10 cytokines which showed the highest concentration levels. Statistical significance of changes in infected compared to non-infected cells was calculated by one-way ANOVA and Tukey's multiple comparison test. The asterisks indicate statistically significant results. ** p = 0.001 to 0.01, *** p < 0.001.

Article Snippet: Cell culture supernatants were screened with the Proteome ProfilerTM Human Cytokine Antibody Array, Panel A (R&D Systems).

Techniques: Infection, Quantitative Proteomics, Cell Culture, Protein Array, Positive Control, Concentration Assay, Comparison

Journal: Virus Research

Article Title: Cowpox virus but not Vaccinia virus induces secretion of CXCL1, IL-8 and IL-6 and chemotaxis of monocytes in vitro

doi: 10.1016/j.virusres.2012.11.013

Figure Lengend Snippet: Differential regulation of human cytokine secretion by VACV or CPXV infection.

Article Snippet: Cell culture supernatants were screened with the Proteome ProfilerTM Human Cytokine Antibody Array, Panel A (R&D Systems).

Techniques: Infection